Structural and functional analysis of the S proteins of two human coronavirus OC43 strains adapted to growth in different cells.
Identifieur interne : 006530 ( Main/Exploration ); précédent : 006529; suivant : 006531Structural and functional analysis of the S proteins of two human coronavirus OC43 strains adapted to growth in different cells.
Auteurs : F. Künkel [Allemagne] ; G. HerrlerSource :
- Archives of virology [ 0304-8608 ] ; 1996.
Descripteurs français
- KwdFr :
- ADN viral, ARN viral, Acides sialiques (métabolisme), Adaptation physiologique, Animaux, Bovins, Cellules Vero, Chiens, Coronavirus (croissance et développement), Coronavirus (génétique), Coronavirus bovin (croissance et développement), Coronavirus bovin (génétique), Coronavirus humain OC43, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (physiologie), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (physiologie), Récepteurs viraux (métabolisme), Similitude de séquences d'acides aminés, Souris, Séquence d'acides aminés, Séquence nucléotidique.
- MESH :
- croissance et développement : Coronavirus, Coronavirus bovin.
- génétique : Coronavirus, Coronavirus bovin, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- métabolisme : Acides sialiques, Récepteurs viraux.
- physiologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- ADN viral, ARN viral, Adaptation physiologique, Animaux, Bovins, Cellules Vero, Chiens, Coronavirus humain OC43, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Similitude de séquences d'acides aminés, Souris, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Adaptation, Physiological, Amino Acid Sequence, Animals, Base Sequence, Cattle, Cell Line, Chlorocebus aethiops, Coronavirus (genetics), Coronavirus (growth & development), Coronavirus OC43, Human, Coronavirus, Bovine (genetics), Coronavirus, Bovine (growth & development), DNA, Viral, Dogs, Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (physiology), Mice, Molecular Sequence Data, RNA, Viral, Receptors, Virus (metabolism), Sequence Homology, Amino Acid, Sialic Acids (metabolism), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (physiology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Receptors, Virus, Sialic Acids.
- chemical , physiology : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical : DNA, Viral, RNA, Viral, Spike Glycoprotein, Coronavirus.
- genetics : Coronavirus, Coronavirus, Bovine.
- growth & development : Coronavirus, Coronavirus, Bovine.
- Adaptation, Physiological, Amino Acid Sequence, Animals, Base Sequence, Cattle, Cell Line, Chlorocebus aethiops, Coronavirus OC43, Human, Dogs, Humans, Mice, Molecular Sequence Data, Sequence Homology, Amino Acid, Vero Cells.
Abstract
The receptor-binding activity of strain CU (grown in MDCK I cells) and of strain VA (adapted to Vero cells) of human coronavirus OC43 was analyzed and compared with the binding activity of bovine coronavirus (BCV) and of the OC43 strain provided by the American Type Culture Collection (AT). Results obtained with resialylated erythrocytes indicated that the ability of the viruses to recognize 9-O-acetylated sialic acid in an alpha 2,6-linkage decreased in the following order: AT > CU > BCV > VA. Only minor differences were observed with respect to the alpha 2,3-linkage. The amino acid sequence of the S protein of strain CU and VA was derived from the nucleotide sequence of the cloned gene. Strain VA differed from strain CU in 34 positions, 18 in the S1 and 16 in the S2 subunit.
DOI: 10.1007/bf01718615
PubMed: 8712929
Affiliations:
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Le document en format XML
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Amino Acid</term><term>Sialic Acids (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term><term>Acides sialiques (métabolisme)</term><term>Adaptation physiologique</term><term>Animaux</term><term>Bovins</term><term>Cellules Vero</term><term>Chiens</term><term>Coronavirus (croissance et développement)</term><term>Coronavirus (génétique)</term><term>Coronavirus bovin (croissance et développement)</term><term>Coronavirus bovin (génétique)</term><term>Coronavirus humain OC43</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (physiologie)</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines de l'enveloppe virale 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Sequence</term><term>Cattle</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Coronavirus OC43, Human</term><term>Dogs</term><term>Humans</term><term>Mice</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term><term>Adaptation physiologique</term><term>Animaux</term><term>Bovins</term><term>Cellules Vero</term><term>Chiens</term><term>Coronavirus humain OC43</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Similitude de séquences d'acides aminés</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The receptor-binding activity of strain CU (grown in MDCK I cells) and of strain VA (adapted to Vero cells) of human coronavirus OC43 was analyzed and compared with the binding activity of bovine coronavirus (BCV) and of the OC43 strain provided by the American Type Culture Collection (AT). Results obtained with resialylated erythrocytes indicated that the ability of the viruses to recognize 9-O-acetylated sialic acid in an alpha 2,6-linkage decreased in the following order: AT > CU > BCV > VA. Only minor differences were observed with respect to the alpha 2,3-linkage. The amino acid sequence of the S protein of strain CU and VA was derived from the nucleotide sequence of the cloned gene. Strain VA differed from strain CU in 34 positions, 18 in the S1 and 16 in the S2 subunit.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Herrler, G" sort="Herrler, G" uniqKey="Herrler G" first="G" last="Herrler">G. Herrler</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Kunkel, F" sort="Kunkel, F" uniqKey="Kunkel F" first="F" last="Künkel">F. Künkel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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